Abstract
This article uses a real-life example to illustrate the concept and methodology of recombineering, a revolutionary genetic engineering technique based on phage-mediated homologous recombination. A step-by-step approach is presented along with a flow diagram, from obtaining gene-harboring BACs to the in vitro generation of a conditional null allele. This method can be used to target any gene at any position, without the knowledge or use of any restriction site. The extensive applicability of recombineering to gene manipulation is discussed.
Original language | English |
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Pages (from-to) | 83-91 |
Number of pages | 9 |
Journal | Frontiers in Biology |
Volume | 7 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2012 |
Keywords
- gene manipulation
- odf2
- recombineering