TY - CHAP
T1 - Troubleshooting and challenges of Next-generation sequencing technology in forensic use
AU - D'Orio, Eugenio
AU - Toso, Aurora
AU - Montagna, Paola
AU - Gemmellaro, Maria Denise
N1 - Publisher Copyright:
© 2024 Elsevier Inc. All rights reserved, including those for text and data mining, AI training, and similar technologies.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Before the implementation of Next-generation sequencing (NGS) technology into forensic practice and prior to its use in casework, its performance needs to be validated. Validation studies are needed so that procedural limitations are defined, and to prove the robustness of the method, its repeatability and reliability. Once the process of validation is completed, standard operating procedures and interpretation guidelines can be established, and NGS can be used routinely in the laboratory workflow. The FBI updated the Quality Assurance Standards for forensic DNA testing laboratories in 2020. Laboratories performing forensic DNA testing or utilizing the Combined DNA Index System must follow these standards to ensure the quality and integrity of the data generated. The Scientific Working Group on DNA Analysis Methods also updated their validation and interpretation guidelines to address NGS technologies. These documents are meant to assist forensic laboratories in the process of validation and implementation of NGS workflows.
AB - Before the implementation of Next-generation sequencing (NGS) technology into forensic practice and prior to its use in casework, its performance needs to be validated. Validation studies are needed so that procedural limitations are defined, and to prove the robustness of the method, its repeatability and reliability. Once the process of validation is completed, standard operating procedures and interpretation guidelines can be established, and NGS can be used routinely in the laboratory workflow. The FBI updated the Quality Assurance Standards for forensic DNA testing laboratories in 2020. Laboratories performing forensic DNA testing or utilizing the Combined DNA Index System must follow these standards to ensure the quality and integrity of the data generated. The Scientific Working Group on DNA Analysis Methods also updated their validation and interpretation guidelines to address NGS technologies. These documents are meant to assist forensic laboratories in the process of validation and implementation of NGS workflows.
KW - Developmental validation
KW - FBI quality assurance standards
KW - Internal validation
KW - SWGDAM guidelines
KW - Validation studies
UR - http://www.scopus.com/inward/record.url?scp=85183216488&partnerID=8YFLogxK
U2 - 10.1016/B978-0-323-99144-5.00006-8
DO - 10.1016/B978-0-323-99144-5.00006-8
M3 - Chapter
AN - SCOPUS:85183216488
SN - 9780323993807
SP - 471
EP - 484
BT - Next Generation Sequencing (NGS) Technology in DNA Analysis
PB - Elsevier
ER -