TY - JOUR
T1 - The levels of boron-uptake proteins in roots are correlated with tolerance to boron stress in barley
AU - Mishra, Sasmita
AU - Heckathorn, Scott
AU - Krause, Charles
N1 - Publisher Copyright:
© Crop Science Society of America.
PY - 2015
Y1 - 2015
N2 - Boron (B) is an essential micronutrient required for plant growth and development. Recently, two major B-uptake proteins, BOR 1 and NIP5;1, have been identified and partially characterized. BOR 1 is a high-affinity B transporter involved in xylem loading in roots, and NIP5;1 is a major boric-acid channel in the plasma membrane. The aim of the present study was to determine if plant tolerance to B stress (deficiency or toxicity) is correlated with natural levels of B-uptake proteins in roots. We grew two barley (Hordeum vulgare L.) cultivars that differ in tolerance to low and high B (Schooner and Clipper, respectively) at three B levels (sub-, near-, and supraoptimal), and then we determined the concentration of BOR 1 and NIP5;1 in roots using enzyme-linked immunosorbent assay (ELISA) and proteinspecific antibodies that we developed. Shoot and root biomass in Schooner decreased at high B, while shoot mass in Clipper decreased in low B. Differences between cultivars in tolerance to B stress were unrelated to the concentration of B in plant tissue or to effects of B on root total protein concentration. BOR 1 content per unit total protein and per gram of root was greater in Clipper than Schooner, while NIP5;1 content was greater in Schooner. The estimated activity of BOR 1 (B uptake per BOR 1) did not differ between cultivars. These results show that whole-plant tolerance to B deficiency and toxicity is correlated with levels of the major B-uptake proteins in roots (BOR 1 and NIP5;1). Hence, BOR 1 and NIP5;1 can potentially be used as biomarkers to identify plant genotypes with enhanced tolerance to B stress (e.g., for agriculture or phytoremediation).
AB - Boron (B) is an essential micronutrient required for plant growth and development. Recently, two major B-uptake proteins, BOR 1 and NIP5;1, have been identified and partially characterized. BOR 1 is a high-affinity B transporter involved in xylem loading in roots, and NIP5;1 is a major boric-acid channel in the plasma membrane. The aim of the present study was to determine if plant tolerance to B stress (deficiency or toxicity) is correlated with natural levels of B-uptake proteins in roots. We grew two barley (Hordeum vulgare L.) cultivars that differ in tolerance to low and high B (Schooner and Clipper, respectively) at three B levels (sub-, near-, and supraoptimal), and then we determined the concentration of BOR 1 and NIP5;1 in roots using enzyme-linked immunosorbent assay (ELISA) and proteinspecific antibodies that we developed. Shoot and root biomass in Schooner decreased at high B, while shoot mass in Clipper decreased in low B. Differences between cultivars in tolerance to B stress were unrelated to the concentration of B in plant tissue or to effects of B on root total protein concentration. BOR 1 content per unit total protein and per gram of root was greater in Clipper than Schooner, while NIP5;1 content was greater in Schooner. The estimated activity of BOR 1 (B uptake per BOR 1) did not differ between cultivars. These results show that whole-plant tolerance to B deficiency and toxicity is correlated with levels of the major B-uptake proteins in roots (BOR 1 and NIP5;1). Hence, BOR 1 and NIP5;1 can potentially be used as biomarkers to identify plant genotypes with enhanced tolerance to B stress (e.g., for agriculture or phytoremediation).
UR - http://www.scopus.com/inward/record.url?scp=84930910916&partnerID=8YFLogxK
U2 - 10.2135/cropsci2014.10.0706
DO - 10.2135/cropsci2014.10.0706
M3 - Article
AN - SCOPUS:84930910916
SN - 0011-183X
VL - 55
SP - 1741
EP - 1748
JO - Crop Science
JF - Crop Science
IS - 4
ER -