The effect of boron availability, CO2, and irradiance on relative accumulation of the major boron transport proteins, BOR1 and NIP5;1

S. Mishra, S. A. Heckathorn, J. M. Frantz, C. Krause

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Boron (B) is an essential plant micronutrient. Two major B-transport proteins have been recently identified and partially characterized: BOR1, a high-affinity B efflux transporter involved in xylem loading, and NIP5;1, a plasma-membrane boric-acid channel involved in B uptake. To date, studies of these B transporters have investigated their expression individually (mainly as mRNA), and only in response to variation in B availability (mostly B deficiency); the influence of other factors, such as plant resource status, has not been studied. To address this, we grew geranium (Pelargonium × hortorum cv. Maverick White) plants under ambient or elevated CO2 concentration, different sub-saturating irradiances, and different B availability. For comparison we also grew three other species (Arabidopsis thaliana, Azolla caroliniana, and Hordeum vulgare) under broad range of B supply. Relative accumulation of BOR1 and NIP5;1 proteins were measured using protein-specific antibodies and Western blotting or ELISA. Elevated CO2 significantly increased content of NIP5;1, while increases in irradiance increased BOR1 content, but decreased NIP5;1 content. Across species, content of both transporters often decreased with increasing B availability, but sometimes remained unchanged or even increased, depending on CO2, irradiance, species, or transporter. Content of BOR1 and NIP5;1 was correlated with root proteins, B content, and sugar content (for high CO2 only), as well as B uptake, but CO2 and irradiance often affected these relationships. Thus, relative accumulation of BOR1 and NIP5;1 is influenced not only by B content, as expected, but by other environmental factors as well.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalBiologia Plantarum
Volume62
Issue number1
DOIs
StatePublished - 1 Jan 2018

Keywords

  • Arabidopsis thaliana
  • Azolla caroliniana
  • B uptake
  • Hordeum vulgare
  • Pelargonium × hortorum
  • photosynthesis

Fingerprint

Dive into the research topics of 'The effect of boron availability, CO2, and irradiance on relative accumulation of the major boron transport proteins, BOR1 and NIP5;1'. Together they form a unique fingerprint.

Cite this