TY - JOUR
T1 - Study of tyrosine hydroxylase immunoreactive neurons in neonate rats lactationally exposed to 2,4-dichlorophenoxyacetic acid
AU - Garcia, G.
AU - Tagliaferro, P.
AU - Ferri, A.
AU - Evangelista De Duffard, A. M.
AU - Duffard, R.
AU - Brusco, A.
PY - 2004/12
Y1 - 2004/12
N2 - Dopaminergic neurons from the midbrain nuclei substantia nigra (SN; A9) and ventral tegmental area (VTA; A10) were investigated by tyrosine hydroxylase (TH) immunostaining in neonate rat brains exposed to 2,4-dichlorophenoxyacetic acid (2,4-D) through lactation. Dorsal raphe serotonin (5-HT) projections to SN and VTA were also studied by 5-HT transporter (5-HTT) immunostaining and results were quantified by image analysis. Twenty-five-day-old pups exposed to 2,4-D through mothers milk were used. Dams were intraperitoneally administered 70 or 100 mg/kg/day of 2,4-D from the 9th to the 25th postpartum day. After 100 mg/kg of 2,4-D exposure, a 25% diminution in the SN and a 33% diminution in the VTA neurons' TH immunostaining along with a significantly 5-HT fiber density diminution were observed. The present work supports previous reports which suggest that exposure to 2,4-D during development has multiple effects on CNS.
AB - Dopaminergic neurons from the midbrain nuclei substantia nigra (SN; A9) and ventral tegmental area (VTA; A10) were investigated by tyrosine hydroxylase (TH) immunostaining in neonate rat brains exposed to 2,4-dichlorophenoxyacetic acid (2,4-D) through lactation. Dorsal raphe serotonin (5-HT) projections to SN and VTA were also studied by 5-HT transporter (5-HTT) immunostaining and results were quantified by image analysis. Twenty-five-day-old pups exposed to 2,4-D through mothers milk were used. Dams were intraperitoneally administered 70 or 100 mg/kg/day of 2,4-D from the 9th to the 25th postpartum day. After 100 mg/kg of 2,4-D exposure, a 25% diminution in the SN and a 33% diminution in the VTA neurons' TH immunostaining along with a significantly 5-HT fiber density diminution were observed. The present work supports previous reports which suggest that exposure to 2,4-D during development has multiple effects on CNS.
KW - 2,4-Dichlorophenoxyacetic acid
KW - Dopaminergic neurons
KW - Serotonin transporter
KW - Substantia nigra
KW - Ventral tegmental area
UR - http://www.scopus.com/inward/record.url?scp=5044231139&partnerID=8YFLogxK
U2 - 10.1016/j.neuro.2004.05.004
DO - 10.1016/j.neuro.2004.05.004
M3 - Article
C2 - 15474613
AN - SCOPUS:5044231139
SN - 0161-813X
VL - 25
SP - 951
EP - 957
JO - NeuroToxicology
JF - NeuroToxicology
IS - 6
ER -