Abstract
Many AB toxins contain an enzymatic A moiety that is anchored to a cell-binding B moiety by a disulfidebridge. After receptor-mediated endocytosis, some AB toxins undergo retrograde transport to the endoplasmic reticulum (ER) where reduction of the disulfidebond occurs. The reduced A subunit then dissociates from the holotoxin and enters the cytosol to alter its cellular target. Intoxication requires A chain separation from the holotoxin, but, for many toxins, it is unclear if reduction alone is sufficientfor toxin disassembly. Here, we examined the link between reduction and disassembly for several ER-translocating toxins. We found disassembly of the reduced Escherichia coli heat-labile enterotoxin (Ltx) required an interaction with one specificER-localized oxidoreductase: protein disulfideisomerase (PDI). In contrast, the reduction and disassembly of ricin toxin (Rtx) and Shiga toxin 1 (Stx1) were coupled events that did not require PDI and could be triggered by reductant alone. PDI-deficientcells accordingly exhibited high resistance to Ltx with continued sensitivity to Rtx and Stx1. The distinct structural organization of each AB toxin thus appears to determine whether holotoxin disassembly occurs spontaneously upon disulfidereduction or requires the additional input of PDI.
Original language | English |
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Journal | Infection and Immunity |
Volume | 91 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2023 |
Keywords
- AB toxin
- ERp57
- ERp72
- protein disulfideisomerase
- Shiga toxin 2
- surface plasmon resonance