Proteomics of Synechocystis sp. PCC 6803: Identification of novel integral plasma membrane proteins

Tatiana Pisareva, Maria Shumskaya, Gianluca Maddalo, Leopold Ilag, Birgitta Norling

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

The cyanobacterial plasma membrane is an essential cell barrier with functions such as the control of taxis, nutrient uptake and secretion. These functions are carried out by integral membrane proteins, which are difficult to identify using standard proteomic methods. In this study, integral proteins were enriched from purified plasma membranes of Synechocystis sp. PCC 6803 using urea wash followed by protein resolution in 1D SDS/PAGE. In total, 51 proteins were identified by peptide mass fingerprinting using MALDI-TOF MS. More than half of the proteins were predicted to be integral with 1-12 transmembrane helices. The majority of the proteins had not been identified previously, and include members of metalloproteases, chemotaxis proteins, secretion proteins, as well as type 2 NAD(P)H dehydrogenase and glycosyltransferase. The obtained results serve as a useful reference for further investigations of the address codes for targeting of integral membrane proteins in cyanobacteria.

Original languageEnglish
Pages (from-to)791-804
Number of pages14
JournalFEBS Journal
Volume274
Issue number3
DOIs
StatePublished - Feb 2007

Keywords

  • Cyanobacteria
  • Integral proteins
  • Plasma membrane
  • Proteome
  • Synechocystis 6803

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