TY - JOUR
T1 - Proteomic analysis of Arabidopsis thaliana leaves in response to acute boron deficiency and toxicity reveals effects on photosynthesis, carbohydrate metabolism, and protein synthesis
AU - Chen, Mei
AU - Mishra, Sasmita
AU - Heckathorn, Scott A.
AU - Frantz, Jonathan M.
AU - Krause, Charles
PY - 2014/2/15
Y1 - 2014/2/15
N2 - Boron (B) stress (deficiency and toxicity) is common in plants, but as the functions of this essential micronutrient are incompletely understood, so too are the effects of B stress. To investigate mechanisms underlying B stress, we examined protein profiles in leaves of Arabidopsis thaliana plants grown under normal B (30. μM), compared to plants transferred for 60 and 84. h (i.e., before and after initial visible symptoms) in deficient (0. μM) or toxic (3. mM) levels of B. B-responsive polypeptides were sequenced by mass spectrometry, following 2D gel electrophoresis, and 1D gels and immunoblotting were used to confirm the B-responsiveness of some of these proteins. Fourteen B-responsive proteins were identified, including: 9 chloroplast proteins, 6 proteins of photosynthetic/carbohydrate metabolism (rubisco activase, OEC23, photosystem I reaction center subunit II-1, ATPase δ-subunit, glycolate oxidase, fructose bisphosphate aldolase), 6 stress proteins, and 3 proteins involved in protein synthesis (note that the 14 proteins may fall into multiple categories). Most (8) of the B-responsive proteins decreased under both B deficiency and toxicity; only 3 increased with B stress. Boron stress decreased, or had no effect on, 3 of 4 oxidative stress proteins examined, and did not affect total protein. Hence, our results indicate relatively early specific effects of B stress on chloroplasts and protein synthesis.
AB - Boron (B) stress (deficiency and toxicity) is common in plants, but as the functions of this essential micronutrient are incompletely understood, so too are the effects of B stress. To investigate mechanisms underlying B stress, we examined protein profiles in leaves of Arabidopsis thaliana plants grown under normal B (30. μM), compared to plants transferred for 60 and 84. h (i.e., before and after initial visible symptoms) in deficient (0. μM) or toxic (3. mM) levels of B. B-responsive polypeptides were sequenced by mass spectrometry, following 2D gel electrophoresis, and 1D gels and immunoblotting were used to confirm the B-responsiveness of some of these proteins. Fourteen B-responsive proteins were identified, including: 9 chloroplast proteins, 6 proteins of photosynthetic/carbohydrate metabolism (rubisco activase, OEC23, photosystem I reaction center subunit II-1, ATPase δ-subunit, glycolate oxidase, fructose bisphosphate aldolase), 6 stress proteins, and 3 proteins involved in protein synthesis (note that the 14 proteins may fall into multiple categories). Most (8) of the B-responsive proteins decreased under both B deficiency and toxicity; only 3 increased with B stress. Boron stress decreased, or had no effect on, 3 of 4 oxidative stress proteins examined, and did not affect total protein. Hence, our results indicate relatively early specific effects of B stress on chloroplasts and protein synthesis.
KW - B-deficiency
KW - B-toxicity
KW - Photosynthesis
UR - http://www.scopus.com/inward/record.url?scp=84894936813&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2013.07.008
DO - 10.1016/j.jplph.2013.07.008
M3 - Article
C2 - 23988561
AN - SCOPUS:84894936813
SN - 0176-1617
VL - 171
SP - 235
EP - 242
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 3-4
ER -