Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220

Maria A. Sinetova; Kenzhegul Bolatkhan; Roman A. Sidorov; Kirill S. Mironov; Alexandra N. Skrypnik; Elena V. Kupriyanova; Bolatkhan K. Zayadan; Maria Shumskaya; Dmitry A. Los

doi:10.1093/femsle/fnx027

Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220

Maria A. Sinetova
, Kenzhegul Bolatkhan
, Roman A. Sidorov
, Kirill S. Mironov
, Alexandra N. Skrypnik
, Elena V. Kupriyanova
, Bolatkhan K. Zayadan
, Maria Shumskaya
, Dmitry A. Los

Biology

Research output: Contribution to journal › Letter › peer-review

24 Scopus citations

Abstract

A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ⁷ and 16:2Δ^7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ⁷ and 16:2Δ^7,10 fatty acids in Desertifilum is proposed.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	FEMS Microbiology Letters
Volume	364
Issue number	4
DOIs	https://doi.org/10.1093/femsle/fnx027
State	Published - 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 15 Life on Land

Keywords

Desertifilum
Fatty acid desaturases
Fatty acids
Molecular phylogeny
Polyphasic approach
Ultrastructure

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10.1093/femsle/fnx027

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title = "Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220",

abstract = "A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ7 and 16:2Δ7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ7 and 16:2Δ7,10 fatty acids in Desertifilum is proposed.",

keywords = "Desertifilum, Fatty acid desaturases, Fatty acids, Molecular phylogeny, Polyphasic approach, Ultrastructure",

author = "Sinetova, \{Maria A.\} and Kenzhegul Bolatkhan and Sidorov, \{Roman A.\} and Mironov, \{Kirill S.\} and Skrypnik, \{Alexandra N.\} and Kupriyanova, \{Elena V.\} and Zayadan, \{Bolatkhan K.\} and Maria Shumskaya and Los, \{Dmitry A.\}",

note = "Publisher Copyright: {\textcopyright} FEMS 2017.",

year = "2017",

doi = "10.1093/femsle/fnx027",

language = "English",

volume = "364",

pages = "1--10",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "4",

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TY - JOUR

T1 - Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220

AU - Sinetova, Maria A.

AU - Bolatkhan, Kenzhegul

AU - Sidorov, Roman A.

AU - Mironov, Kirill S.

AU - Skrypnik, Alexandra N.

AU - Kupriyanova, Elena V.

AU - Zayadan, Bolatkhan K.

AU - Shumskaya, Maria

AU - Los, Dmitry A.

N1 - Publisher Copyright: © FEMS 2017.

PY - 2017

Y1 - 2017

N2 - A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ7 and 16:2Δ7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ7 and 16:2Δ7,10 fatty acids in Desertifilum is proposed.

AB - A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ7 and 16:2Δ7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ7 and 16:2Δ7,10 fatty acids in Desertifilum is proposed.

KW - Desertifilum

KW - Fatty acid desaturases

KW - Fatty acids

KW - Molecular phylogeny

KW - Polyphasic approach

KW - Ultrastructure

UR - https://www.scopus.com/pages/publications/85019903644

U2 - 10.1093/femsle/fnx027

DO - 10.1093/femsle/fnx027

M3 - Letter

C2 - 28130365

AN - SCOPUS:85019903644

SN - 0378-1097

VL - 364

SP - 1

EP - 10

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 4

ER -

Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220

Abstract

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Keywords

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