TY - JOUR
T1 - Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220
AU - Sinetova, Maria A.
AU - Bolatkhan, Kenzhegul
AU - Sidorov, Roman A.
AU - Mironov, Kirill S.
AU - Skrypnik, Alexandra N.
AU - Kupriyanova, Elena V.
AU - Zayadan, Bolatkhan K.
AU - Shumskaya, Maria
AU - Los, Dmitry A.
N1 - Publisher Copyright:
© FEMS 2017.
PY - 2017
Y1 - 2017
N2 - A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ7 and 16:2Δ7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ7 and 16:2Δ7,10 fatty acids in Desertifilum is proposed.
AB - A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ7 and 16:2Δ7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ7 and 16:2Δ7,10 fatty acids in Desertifilum is proposed.
KW - Desertifilum
KW - Fatty acid desaturases
KW - Fatty acids
KW - Molecular phylogeny
KW - Polyphasic approach
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=85019903644&partnerID=8YFLogxK
U2 - 10.1093/femsle/fnx027
DO - 10.1093/femsle/fnx027
M3 - Letter
C2 - 28130365
AN - SCOPUS:85019903644
SN - 0378-1097
VL - 364
SP - 1
EP - 10
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 4
ER -