TY - JOUR
T1 - Morphometric study on cytoskeletal components of neuronal and astroglial cells after chronic CB1 agonist treatment.
AU - Tagliaferro, Patricia
AU - Ramos, Alberto J.
AU - Onaivi, Emmanuel S.
AU - Evrard, Sergio G.
AU - Vega, Maite Duhalde
AU - Brusco, Alicia
PY - 2006
Y1 - 2006
N2 - One of the major goals for the use of digital image analysis systems in neuroanatomy is to visualize structures, cells, or other tissue components in order to compare various populations. In addition, digital image analysis allows semi-quantification of cell labeling because it is capable of measuring simultaneously the staining intensity, location, size, and shape of labeled profiles. In the present work, the morphological changes in the CB1 hippocampal area and corpus striatum induced by chronic treatment with the synthetic CB1-receptor agonist WIN55,212-2 were analyzed as an example of digital image analysis application. Twice-daily treatment for 14 d with the CB1-receptor agonist demonstrated significant changes in the expression of neuronal cytoskeletal proteins and in neuronal morphology, as evidenced by immunocytochemical and digital analysis studies. However, changes in the expression of astroglial cytoskeletal proteins were not found.
AB - One of the major goals for the use of digital image analysis systems in neuroanatomy is to visualize structures, cells, or other tissue components in order to compare various populations. In addition, digital image analysis allows semi-quantification of cell labeling because it is capable of measuring simultaneously the staining intensity, location, size, and shape of labeled profiles. In the present work, the morphological changes in the CB1 hippocampal area and corpus striatum induced by chronic treatment with the synthetic CB1-receptor agonist WIN55,212-2 were analyzed as an example of digital image analysis application. Twice-daily treatment for 14 d with the CB1-receptor agonist demonstrated significant changes in the expression of neuronal cytoskeletal proteins and in neuronal morphology, as evidenced by immunocytochemical and digital analysis studies. However, changes in the expression of astroglial cytoskeletal proteins were not found.
UR - http://www.scopus.com/inward/record.url?scp=33646827446&partnerID=8YFLogxK
M3 - Article
C2 - 16506403
AN - SCOPUS:33646827446
SN - 1543-1894
VL - 123
SP - 91
EP - 104
JO - Methods in molecular medicine
JF - Methods in molecular medicine
ER -