Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio)

Maria Rayane Correia de Oliveira; Sacha Aubrey Alves Rodrigues Santos; Gabriela Alves do Nascimento; João Gabriel Leite da Silva; Luiz Francisco Wemmenson Gonçalves Moura; Paulo Adenes Teixeira Coelho; Lorena Silva Lima; Keciany Alves de Oliveira; Francisco Lucas Alves Batista; Daniela Braga de Sousa; Francisco Bastos Cavalcante Sobrinho; Sandra Maria Barbosa de Araújo; Antonio Gustavo de Almeida Batista; Djane Ventura de Azevedo; Franciglauber Silva Bezerra; Larissa Morais Ribeiro da Silva; Maria Izabel Florindo Guedes; Henrique Douglas Melo Coutinho; Renalison Farias-Pereira; Ramon da Silva Raposo; Adriana Rolim Campos; Francisco Ernani Alves Magalhães

doi:10.1016/j.vascn.2025.107748

Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio)

Maria Rayane Correia de Oliveira, Sacha Aubrey Alves Rodrigues Santos, Gabriela Alves do Nascimento, João Gabriel Leite da Silva, Luiz Francisco Wemmenson Gonçalves Moura, Paulo Adenes Teixeira Coelho, Lorena Silva Lima, Keciany Alves de Oliveira, Francisco Lucas Alves Batista, Daniela Braga de Sousa, Francisco Bastos Cavalcante Sobrinho, Sandra Maria Barbosa de Araújo, Antonio Gustavo de Almeida Batista, Djane Ventura de Azevedo, Franciglauber Silva Bezerra, Larissa Morais Ribeiro da Silva, Maria Izabel Florindo Guedes, Henrique Douglas Melo Coutinho, Renalison Farias-Pereira, Ramon da Silva RaposoAdriana Rolim Campos, Francisco Ernani Alves Magalhães

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

In this study a behavioral model of LPS-induced abdominal nociception was developed on adult zebrafish (Danio rerio), aZF. Initially, the toxicity of different LPS concentrations was assessed. The abdominal nociceptive response to the lowest LPS concentration was then analyzed, and the impact of sex on this nociceptive response was evaluated. The behavioral model of abdominal nociception was defined using the antinociceptive effects of morphine and diclofenac sodium. The mechanism of the possible involvement of TRPA1 in LPS-induced abdominal nociception was evaluated using HC-030031. Additionally, was investigated whether naloxone could modulate morphine's antinociceptive effect. The Light & Dark Test was conducted to assess any potential anxiolytic-like effect of LPS. The Open Field Test was performed to evaluate the possible sedative effect/or not of morphine and diclofenac sodium. As a result, the tested LPS endotoxin solutions were not endotoxic against aZF (LC₅₀>0.25 mg/mL). LPS significantly increased the abdominal nociceptive behavior of aZF. Sex did not affect the response profile to the endotoxin. Morphine and diclofenac sodium inhibited abdominal nociception induced by LPS and the effect of morphine was blocked by naloxone. HC-030031 significantly inhibited abdominal nociception induced by LPS. The LPS did not cause an anxiolytic-like effect. Morphine and diclofenac sodium did not affect the locomotion of the animals. The results suggest that aZF can be used as a behavioral model of abdominal nociception induced by LPS endotoxin.

Original language	English
Article number	107748
Journal	Journal of Pharmacological and Toxicological Methods
Volume	135
DOIs	https://doi.org/10.1016/j.vascn.2025.107748
State	Published - Sep 2025

Keywords

Abdominal pain
Adult zebrafish (Danio rerio)
Bacterial endotoxin LPS
Behavioral model
TRPA1

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10.1016/j.vascn.2025.107748

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de Oliveira, M. R. C., Santos, S. A. A. R., do Nascimento, G. A., da Silva, J. G. L., Moura, L. F. W. G., Coelho, P. A. T., Lima, L. S., de Oliveira, K. A., Batista, F. L. A., de Sousa, D. B., Cavalcante Sobrinho, F. B., de Araújo, S. M. B., Batista, A. G. D. A., de Azevedo, D. V., Bezerra, F. S., da Silva, L. M. R., Guedes, M. I. F., Coutinho, H. D. M., Farias-Pereira, R., ... Magalhães, F. E. A. (2025). Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio). Journal of Pharmacological and Toxicological Methods, 135, Article 107748. https://doi.org/10.1016/j.vascn.2025.107748

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title = "Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio)",

abstract = "In this study a behavioral model of LPS-induced abdominal nociception was developed on adult zebrafish (Danio rerio), aZF. Initially, the toxicity of different LPS concentrations was assessed. The abdominal nociceptive response to the lowest LPS concentration was then analyzed, and the impact of sex on this nociceptive response was evaluated. The behavioral model of abdominal nociception was defined using the antinociceptive effects of morphine and diclofenac sodium. The mechanism of the possible involvement of TRPA1 in LPS-induced abdominal nociception was evaluated using HC-030031. Additionally, was investigated whether naloxone could modulate morphine's antinociceptive effect. The Light & Dark Test was conducted to assess any potential anxiolytic-like effect of LPS. The Open Field Test was performed to evaluate the possible sedative effect/or not of morphine and diclofenac sodium. As a result, the tested LPS endotoxin solutions were not endotoxic against aZF (LC50>0.25 mg/mL). LPS significantly increased the abdominal nociceptive behavior of aZF. Sex did not affect the response profile to the endotoxin. Morphine and diclofenac sodium inhibited abdominal nociception induced by LPS and the effect of morphine was blocked by naloxone. HC-030031 significantly inhibited abdominal nociception induced by LPS. The LPS did not cause an anxiolytic-like effect. Morphine and diclofenac sodium did not affect the locomotion of the animals. The results suggest that aZF can be used as a behavioral model of abdominal nociception induced by LPS endotoxin.",

keywords = "Abdominal pain, Adult zebrafish (Danio rerio), Bacterial endotoxin LPS, Behavioral model, TRPA1",

author = "{de Oliveira}, {Maria Rayane Correia} and Santos, {Sacha Aubrey Alves Rodrigues} and {do Nascimento}, {Gabriela Alves} and {da Silva}, {Jo{\~a}o Gabriel Leite} and Moura, {Luiz Francisco Wemmenson Gon{\c c}alves} and Coelho, {Paulo Adenes Teixeira} and Lima, {Lorena Silva} and {de Oliveira}, {Keciany Alves} and Batista, {Francisco Lucas Alves} and {de Sousa}, {Daniela Braga} and {Cavalcante Sobrinho}, {Francisco Bastos} and {de Ara{\'u}jo}, {Sandra Maria Barbosa} and Batista, {Antonio Gustavo de Almeida} and {de Azevedo}, {Djane Ventura} and Bezerra, {Franciglauber Silva} and {da Silva}, {Larissa Morais Ribeiro} and Guedes, {Maria Izabel Florindo} and Coutinho, {Henrique Douglas Melo} and Renalison Farias-Pereira and Raposo, {Ramon da Silva} and Campos, {Adriana Rolim} and Magalh{\~a}es, {Francisco Ernani Alves}",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

month = sep,

doi = "10.1016/j.vascn.2025.107748",

language = "English",

volume = "135",

journal = "Journal of Pharmacological and Toxicological Methods",

issn = "1056-8719",

publisher = "Elsevier Inc.",

}

de Oliveira, MRC, Santos, SAAR, do Nascimento, GA, da Silva, JGL, Moura, LFWG, Coelho, PAT, Lima, LS, de Oliveira, KA, Batista, FLA, de Sousa, DB, Cavalcante Sobrinho, FB, de Araújo, SMB, Batista, AGDA, de Azevedo, DV, Bezerra, FS, da Silva, LMR, Guedes, MIF, Coutinho, HDM, Farias-Pereira, R, Raposo, RDS, Campos, AR & Magalhães, FEA 2025, 'Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio)', Journal of Pharmacological and Toxicological Methods, vol. 135, 107748. https://doi.org/10.1016/j.vascn.2025.107748

TY - JOUR

T1 - Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio)

AU - de Oliveira, Maria Rayane Correia

AU - Santos, Sacha Aubrey Alves Rodrigues

AU - do Nascimento, Gabriela Alves

AU - da Silva, João Gabriel Leite

AU - Moura, Luiz Francisco Wemmenson Gonçalves

AU - Coelho, Paulo Adenes Teixeira

AU - Lima, Lorena Silva

AU - de Oliveira, Keciany Alves

AU - Batista, Francisco Lucas Alves

AU - de Sousa, Daniela Braga

AU - Cavalcante Sobrinho, Francisco Bastos

AU - de Araújo, Sandra Maria Barbosa

AU - Batista, Antonio Gustavo de Almeida

AU - de Azevedo, Djane Ventura

AU - Bezerra, Franciglauber Silva

AU - da Silva, Larissa Morais Ribeiro

AU - Guedes, Maria Izabel Florindo

AU - Coutinho, Henrique Douglas Melo

AU - Farias-Pereira, Renalison

AU - Raposo, Ramon da Silva

AU - Campos, Adriana Rolim

AU - Magalhães, Francisco Ernani Alves

PY - 2025/9

Y1 - 2025/9

N2 - In this study a behavioral model of LPS-induced abdominal nociception was developed on adult zebrafish (Danio rerio), aZF. Initially, the toxicity of different LPS concentrations was assessed. The abdominal nociceptive response to the lowest LPS concentration was then analyzed, and the impact of sex on this nociceptive response was evaluated. The behavioral model of abdominal nociception was defined using the antinociceptive effects of morphine and diclofenac sodium. The mechanism of the possible involvement of TRPA1 in LPS-induced abdominal nociception was evaluated using HC-030031. Additionally, was investigated whether naloxone could modulate morphine's antinociceptive effect. The Light & Dark Test was conducted to assess any potential anxiolytic-like effect of LPS. The Open Field Test was performed to evaluate the possible sedative effect/or not of morphine and diclofenac sodium. As a result, the tested LPS endotoxin solutions were not endotoxic against aZF (LC50>0.25 mg/mL). LPS significantly increased the abdominal nociceptive behavior of aZF. Sex did not affect the response profile to the endotoxin. Morphine and diclofenac sodium inhibited abdominal nociception induced by LPS and the effect of morphine was blocked by naloxone. HC-030031 significantly inhibited abdominal nociception induced by LPS. The LPS did not cause an anxiolytic-like effect. Morphine and diclofenac sodium did not affect the locomotion of the animals. The results suggest that aZF can be used as a behavioral model of abdominal nociception induced by LPS endotoxin.

AB - In this study a behavioral model of LPS-induced abdominal nociception was developed on adult zebrafish (Danio rerio), aZF. Initially, the toxicity of different LPS concentrations was assessed. The abdominal nociceptive response to the lowest LPS concentration was then analyzed, and the impact of sex on this nociceptive response was evaluated. The behavioral model of abdominal nociception was defined using the antinociceptive effects of morphine and diclofenac sodium. The mechanism of the possible involvement of TRPA1 in LPS-induced abdominal nociception was evaluated using HC-030031. Additionally, was investigated whether naloxone could modulate morphine's antinociceptive effect. The Light & Dark Test was conducted to assess any potential anxiolytic-like effect of LPS. The Open Field Test was performed to evaluate the possible sedative effect/or not of morphine and diclofenac sodium. As a result, the tested LPS endotoxin solutions were not endotoxic against aZF (LC50>0.25 mg/mL). LPS significantly increased the abdominal nociceptive behavior of aZF. Sex did not affect the response profile to the endotoxin. Morphine and diclofenac sodium inhibited abdominal nociception induced by LPS and the effect of morphine was blocked by naloxone. HC-030031 significantly inhibited abdominal nociception induced by LPS. The LPS did not cause an anxiolytic-like effect. Morphine and diclofenac sodium did not affect the locomotion of the animals. The results suggest that aZF can be used as a behavioral model of abdominal nociception induced by LPS endotoxin.

KW - Abdominal pain

KW - Adult zebrafish (Danio rerio)

KW - Bacterial endotoxin LPS

KW - Behavioral model

KW - TRPA1

UR - http://www.scopus.com/inward/record.url?scp=105007106658&partnerID=8YFLogxK

U2 - 10.1016/j.vascn.2025.107748

DO - 10.1016/j.vascn.2025.107748

M3 - Article

C2 - 40350099

AN - SCOPUS:105007106658

SN - 1056-8719

VL - 135

JO - Journal of Pharmacological and Toxicological Methods

JF - Journal of Pharmacological and Toxicological Methods

M1 - 107748

ER -

Lipopolysaccharide-induced abdominal nociception behavioral model in adult zebrafish (Danio rerio)

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Keywords

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