Independent research projects using protein extraction: Affordable ways to inquire, discover and publish for undergraduate students

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Abstract

This article describes how to use protein extraction, quantification, and analysis in the undergraduate teaching laboratory to engage students in inquiry-based, discoverydriven learning. Detailed instructions for obtaining proteins from animal tissues, using BCA assay to quantify the proteins, and data analysis are provided. The experimental procedure requires laboratory equipment and supplies that can be found in most biology teaching labs. Suggestions for successful implementation that can lead to original research published in peer-reviewed journals are outlined.

Original languageEnglish
Pages (from-to)37-39
Number of pages3
JournalAmerican Biology Teacher
Volume72
Issue number1
DOIs
StatePublished - Jan 2010

Keywords

  • BCA assay
  • Independent research
  • Protein extraction
  • Protein quantification
  • Western blot

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