TY - JOUR
T1 - Independent research projects using protein extraction
T2 - Affordable ways to inquire, discover and publish for undergraduate students
AU - Pu, Rongsun
PY - 2010/1
Y1 - 2010/1
N2 - This article describes how to use protein extraction, quantification, and analysis in the undergraduate teaching laboratory to engage students in inquiry-based, discoverydriven learning. Detailed instructions for obtaining proteins from animal tissues, using BCA assay to quantify the proteins, and data analysis are provided. The experimental procedure requires laboratory equipment and supplies that can be found in most biology teaching labs. Suggestions for successful implementation that can lead to original research published in peer-reviewed journals are outlined.
AB - This article describes how to use protein extraction, quantification, and analysis in the undergraduate teaching laboratory to engage students in inquiry-based, discoverydriven learning. Detailed instructions for obtaining proteins from animal tissues, using BCA assay to quantify the proteins, and data analysis are provided. The experimental procedure requires laboratory equipment and supplies that can be found in most biology teaching labs. Suggestions for successful implementation that can lead to original research published in peer-reviewed journals are outlined.
KW - BCA assay
KW - Independent research
KW - Protein extraction
KW - Protein quantification
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=75949089409&partnerID=8YFLogxK
U2 - 10.1525/abt.2010.72.1.9
DO - 10.1525/abt.2010.72.1.9
M3 - Article
AN - SCOPUS:75949089409
SN - 0002-7685
VL - 72
SP - 37
EP - 39
JO - American Biology Teacher
JF - American Biology Teacher
IS - 1
ER -