TY - JOUR
T1 - Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in Synechocystis
AU - Shoumskaya, Maria A.
AU - Paithoonrangsarid, Kalyanee
AU - Kanesaki, Yu
AU - Los, Dmitry A.
AU - Zinchenko, Vladislav V.
AU - Tanticharoen, Morakot
AU - Suzuki, Iwane
AU - Murata, Norio
PY - 2005/6/3
Y1 - 2005/6/3
N2 - In previous studies, we characterized five histidine kinases (Hiks) and the cognate response regulators (Rres) that control the expression of ~70% of the hyperosmotic stress-inducible genes in the cyanobacterium Synechocystis sp. PCC 6803. In the present study, we screened a gene knock-out library of Rres by RNA slot-blot hybridization and with a genome-wide DNA microarray and identified three Hik-Rre systems, namely, Hik33-Rre31, Hik10-Rre3, and Hik16-Hik41-Rre17, as well as another system that included Rre1, that were involved in perception of salt stress and transduction of the signal. We found that these Hik-Rre systems were identical to those that were involved in perception and transduction of the hyperosmotic stress signal. We compared the induction factors of the salt stress- and hyperosmotic stress-inducible genes that are located down-stream of each system and found that these genes responded to the two kinds of stress to different respective extents. In addition, the Hik33-Rre31 system regulated the expression of genes that were specifically induced by hyperosmotic stress, whereas the system that included Rre1 regulated the expression of one or two genes that were specifically induced either by salt stress or by hyperosmotic stress. Our observations suggest that the perception of salt and hyperosmotic stress by the Hik-Rre systems is complex and that salt stress and hyperosmotic stress are perceived as distinct signals by the Hik-Rre systems.
AB - In previous studies, we characterized five histidine kinases (Hiks) and the cognate response regulators (Rres) that control the expression of ~70% of the hyperosmotic stress-inducible genes in the cyanobacterium Synechocystis sp. PCC 6803. In the present study, we screened a gene knock-out library of Rres by RNA slot-blot hybridization and with a genome-wide DNA microarray and identified three Hik-Rre systems, namely, Hik33-Rre31, Hik10-Rre3, and Hik16-Hik41-Rre17, as well as another system that included Rre1, that were involved in perception of salt stress and transduction of the signal. We found that these Hik-Rre systems were identical to those that were involved in perception and transduction of the hyperosmotic stress signal. We compared the induction factors of the salt stress- and hyperosmotic stress-inducible genes that are located down-stream of each system and found that these genes responded to the two kinds of stress to different respective extents. In addition, the Hik33-Rre31 system regulated the expression of genes that were specifically induced by hyperosmotic stress, whereas the system that included Rre1 regulated the expression of one or two genes that were specifically induced either by salt stress or by hyperosmotic stress. Our observations suggest that the perception of salt and hyperosmotic stress by the Hik-Rre systems is complex and that salt stress and hyperosmotic stress are perceived as distinct signals by the Hik-Rre systems.
UR - http://www.scopus.com/inward/record.url?scp=20444367654&partnerID=8YFLogxK
U2 - 10.1074/jbc.M412174200
DO - 10.1074/jbc.M412174200
M3 - Article
C2 - 15805106
AN - SCOPUS:20444367654
SN - 0021-9258
VL - 280
SP - 21531
EP - 21538
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 22
ER -