Hydrogen peroxide participates in perception and transduction of cold stress signal in synechocystis

Pavel V. Fedurayev; Kirill S. Mironov; David A. Gabrielyan; Vladimir S. Bedbenov; Anna A. Zorina; Maria Shumskaya; Dmitry A. Los

doi:10.1093/pcp/pcy067

Hydrogen peroxide participates in perception and transduction of cold stress signal in synechocystis

Pavel V. Fedurayev
, Kirill S. Mironov
, David A. Gabrielyan
, Vladimir S. Bedbenov
, Anna A. Zorina
, Maria Shumskaya
, Dmitry A. Los

Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The double mutant ΔkatG/tpx of cyanobacterium Synechocystis sp. strain PCC 6803, defective in the anti-oxidative enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), is unable to grow in the presence of exogenous H₂O₂. The ΔkatG/tpx mutant is shown to be extremely sensitive to very low concentrations of H₂O₂, especially when intensified with cold stress. Analysis of gene expression in both wild-type and ΔkatG/tpx mutant cells treated by combined cold/oxidative stress revealed that H₂O₂ participates in regulation of expression of cold-responsive genes, affecting either signal perception or transduction. The central role of a transmembrane stress-sensing histidine kinase Hik33 in the cold/oxidative signal transduction pathway is discussed.

Original language	English
Pages (from-to)	1255-1264
Number of pages	10
Journal	Plant and Cell Physiology
Volume	59
Issue number	6
DOIs	https://doi.org/10.1093/pcp/pcy067
State	Published - 1 Jun 2018

Keywords

Cold stress
Hydrogen peroxide
Oxidative stress
ROS
Synechocystis

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10.1093/pcp/pcy067

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@article{1efdfcb68de74ac09de16ef68a7583cc,

title = "Hydrogen peroxide participates in perception and transduction of cold stress signal in synechocystis",

abstract = "The double mutant ΔkatG/tpx of cyanobacterium Synechocystis sp. strain PCC 6803, defective in the anti-oxidative enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), is unable to grow in the presence of exogenous H2O2. The ΔkatG/tpx mutant is shown to be extremely sensitive to very low concentrations of H2O2, especially when intensified with cold stress. Analysis of gene expression in both wild-type and ΔkatG/tpx mutant cells treated by combined cold/oxidative stress revealed that H2O2 participates in regulation of expression of cold-responsive genes, affecting either signal perception or transduction. The central role of a transmembrane stress-sensing histidine kinase Hik33 in the cold/oxidative signal transduction pathway is discussed.",

keywords = "Cold stress, Hydrogen peroxide, Oxidative stress, ROS, Synechocystis",

author = "Fedurayev, \{Pavel V.\} and Mironov, \{Kirill S.\} and Gabrielyan, \{David A.\} and Bedbenov, \{Vladimir S.\} and Zorina, \{Anna A.\} and Maria Shumskaya and Los, \{Dmitry A.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2018. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.",

year = "2018",

month = jun,

day = "1",

doi = "10.1093/pcp/pcy067",

language = "English",

volume = "59",

pages = "1255--1264",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

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}

TY - JOUR

T1 - Hydrogen peroxide participates in perception and transduction of cold stress signal in synechocystis

AU - Fedurayev, Pavel V.

AU - Mironov, Kirill S.

AU - Gabrielyan, David A.

AU - Bedbenov, Vladimir S.

AU - Zorina, Anna A.

AU - Shumskaya, Maria

AU - Los, Dmitry A.

N1 - Publisher Copyright: © The Author(s) 2018. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - The double mutant ΔkatG/tpx of cyanobacterium Synechocystis sp. strain PCC 6803, defective in the anti-oxidative enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), is unable to grow in the presence of exogenous H2O2. The ΔkatG/tpx mutant is shown to be extremely sensitive to very low concentrations of H2O2, especially when intensified with cold stress. Analysis of gene expression in both wild-type and ΔkatG/tpx mutant cells treated by combined cold/oxidative stress revealed that H2O2 participates in regulation of expression of cold-responsive genes, affecting either signal perception or transduction. The central role of a transmembrane stress-sensing histidine kinase Hik33 in the cold/oxidative signal transduction pathway is discussed.

AB - The double mutant ΔkatG/tpx of cyanobacterium Synechocystis sp. strain PCC 6803, defective in the anti-oxidative enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), is unable to grow in the presence of exogenous H2O2. The ΔkatG/tpx mutant is shown to be extremely sensitive to very low concentrations of H2O2, especially when intensified with cold stress. Analysis of gene expression in both wild-type and ΔkatG/tpx mutant cells treated by combined cold/oxidative stress revealed that H2O2 participates in regulation of expression of cold-responsive genes, affecting either signal perception or transduction. The central role of a transmembrane stress-sensing histidine kinase Hik33 in the cold/oxidative signal transduction pathway is discussed.

KW - Cold stress

KW - Hydrogen peroxide

KW - Oxidative stress

KW - ROS

KW - Synechocystis

UR - https://www.scopus.com/pages/publications/85048050387

U2 - 10.1093/pcp/pcy067

DO - 10.1093/pcp/pcy067

M3 - Article

C2 - 29590456

AN - SCOPUS:85048050387

SN - 0032-0781

VL - 59

SP - 1255

EP - 1264

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 6

ER -

Hydrogen peroxide participates in perception and transduction of cold stress signal in synechocystis

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