TY - JOUR
T1 - Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis
AU - Paithoonrangsarid, Kalyanee
AU - Shoumskaya, Maria A.
AU - Kanesaki, Yu
AU - Satoh, Syusei
AU - Tabata, Satoshi
AU - Los, Dmitry A.
AU - Zinchenko, Vladislav V.
AU - Hayashi, Hidenori
AU - Tanticharoen, Morakot
AU - Suzuki, Iwane
AU - Murata, Norio
PY - 2004/12/17
Y1 - 2004/12/17
N2 - Microorganisms respond to hyperosniotic stress via changes in the levels of expression of large numbers of genes. Such responses are essential for acclimation to a new osmotic environment. To identify factors involved in the perception and transduction of signals caused by hyperosmotic stress, we examined the response of Synechocystis sp. PCC 6803, which has proven to be a particularly useful microorganism in similar analyses. We screened knockout libraries of histidine kinases (Hiks) and response regulators (Rres) in Synechocystis by DNA microarray and slot-blot hybridization analyses, and we identified several two-component systems, which we designated Hik-Rre systems, namely, Hik33-Rre31, Hik34-Rre1, and Hik10-Rre3, as well as Hik16-Hik41-Rre17, as the transducers of hyperosmotic stress. We also identified Hik2-Rre1 as a putative additional two-component system. Each individual two-component system regulated the transcription of a specific group of genes that were responsive to hyperosmotic stress.
AB - Microorganisms respond to hyperosniotic stress via changes in the levels of expression of large numbers of genes. Such responses are essential for acclimation to a new osmotic environment. To identify factors involved in the perception and transduction of signals caused by hyperosmotic stress, we examined the response of Synechocystis sp. PCC 6803, which has proven to be a particularly useful microorganism in similar analyses. We screened knockout libraries of histidine kinases (Hiks) and response regulators (Rres) in Synechocystis by DNA microarray and slot-blot hybridization analyses, and we identified several two-component systems, which we designated Hik-Rre systems, namely, Hik33-Rre31, Hik34-Rre1, and Hik10-Rre3, as well as Hik16-Hik41-Rre17, as the transducers of hyperosmotic stress. We also identified Hik2-Rre1 as a putative additional two-component system. Each individual two-component system regulated the transcription of a specific group of genes that were responsive to hyperosmotic stress.
UR - http://www.scopus.com/inward/record.url?scp=19944425793&partnerID=8YFLogxK
U2 - 10.1074/jbc.M410162200
DO - 10.1074/jbc.M410162200
M3 - Article
C2 - 15471853
AN - SCOPUS:19944425793
SN - 0021-9258
VL - 279
SP - 53078
EP - 53086
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -