TY - JOUR
T1 - Exploring DNA in biochemistry lab courses
T2 - DNA barcoding and phylogenetic analysis
AU - Suárez Casanova, Víctor Manuel
AU - Shumskaya, Maria
N1 - Publisher Copyright:
© 2021 International Union of Biochemistry and Molecular Biology
PY - 2021/9/1
Y1 - 2021/9/1
N2 - DNA structure has been leveraged in a variety of facets that allow scientists to perform a range of assays, including ones for identification of species, establishing evolutionary relationships between taxa, or even identifying individuals. Here, we present a DNA barcoding method as practical, hands-on approach that connects several experimental techniques in one sequence to teach the principles behind DNA isolation, purification, PCR, sequencing, and phylogeny analysis. Our set of exercises is designed for a teaching university laboratory setting. The three laboratory class assignments utilize DNA from a mushroom (can be purchased at a supermarket) and provide a pipeline to guide students through the process of identifying an unknown sample, like in many research laboratories. The third assignment can be used as a stand-alone exercise on phylogeny analysis and can be taught remotely. Students explore the theory behind the standard molecular techniques and apply it in a hands-on setting that involves experimental design, sample preparation, and use of hallmark molecular instruments.
AB - DNA structure has been leveraged in a variety of facets that allow scientists to perform a range of assays, including ones for identification of species, establishing evolutionary relationships between taxa, or even identifying individuals. Here, we present a DNA barcoding method as practical, hands-on approach that connects several experimental techniques in one sequence to teach the principles behind DNA isolation, purification, PCR, sequencing, and phylogeny analysis. Our set of exercises is designed for a teaching university laboratory setting. The three laboratory class assignments utilize DNA from a mushroom (can be purchased at a supermarket) and provide a pipeline to guide students through the process of identifying an unknown sample, like in many research laboratories. The third assignment can be used as a stand-alone exercise on phylogeny analysis and can be taught remotely. Students explore the theory behind the standard molecular techniques and apply it in a hands-on setting that involves experimental design, sample preparation, and use of hallmark molecular instruments.
KW - BLAST
KW - laboratory exercise
KW - PCR
KW - phylogeny
KW - Sanger sequencing
UR - http://www.scopus.com/inward/record.url?scp=85107396647&partnerID=8YFLogxK
U2 - 10.1002/bmb.21551
DO - 10.1002/bmb.21551
M3 - Article
C2 - 34096689
AN - SCOPUS:85107396647
SN - 1470-8175
VL - 49
SP - 789
EP - 799
JO - Biochemistry and Molecular Biology Education
JF - Biochemistry and Molecular Biology Education
IS - 5
ER -