TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of the Saccharomycopsis fibuligera glucoamylase expressed from the GLU1 gene in Escherichia coli
AU - Solovicová, Adriana
AU - Gašperík, Juraj
AU - Ševčík, Jozef
AU - Hostinová, Eva
PY - 1997
Y1 - 1997
N2 - The active non-glycosylated glucoamylase, overexpressed from the Saccharomycopsis fibuligera GLU1 gene in Escherichia coli BL21(DE3), has been purified from the solubilized inclusion bodies and then renatured in vitro. Crystals of the recombinant glucoamylase were obtained by vapour diffusion using PEG as precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell dimensions of a = 58.1, b = 87.8 and c = 99.9 Å, and diffract to 1.7 Å resolution. This is the first report of the crystallization of the full-length glucoamylase corresponding to the mature enzyme.
AB - The active non-glycosylated glucoamylase, overexpressed from the Saccharomycopsis fibuligera GLU1 gene in Escherichia coli BL21(DE3), has been purified from the solubilized inclusion bodies and then renatured in vitro. Crystals of the recombinant glucoamylase were obtained by vapour diffusion using PEG as precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell dimensions of a = 58.1, b = 87.8 and c = 99.9 Å, and diffract to 1.7 Å resolution. This is the first report of the crystallization of the full-length glucoamylase corresponding to the mature enzyme.
UR - http://www.scopus.com/inward/record.url?scp=0030732178&partnerID=8YFLogxK
U2 - 10.1107/S0907444997006835
DO - 10.1107/S0907444997006835
M3 - Article
AN - SCOPUS:0030732178
SN - 0907-4449
VL - 53
SP - 782
EP - 783
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 6
ER -