Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Loree C. Heller; Carisa R. Davis; K. Kealy Peak; David Wingfield; Andrew C. Cannons; Philip T. Amuso; Jacqueline Cattan

doi:10.1128/AEM.69.3.1844-1846.2003

Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Loree C. Heller
, Carisa R. Davis
, K. Kealy Peak
, David Wingfield
, Andrew C. Cannons
, Philip T. Amuso
, Jacqueline Cattan

Biology

University of South Florida
Florida Department of Health

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Original language	English
Pages (from-to)	1844-1846
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	69
Issue number	3
DOIs	https://doi.org/10.1128/AEM.69.3.1844-1846.2003
State	Published - 1 Mar 2003

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10.1128/AEM.69.3.1844-1846.2003

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title = "Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR",

abstract = "In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.",

author = "Heller, \{Loree C.\} and Davis, \{Carisa R.\} and Peak, \{K. Kealy\} and David Wingfield and Cannons, \{Andrew C.\} and Amuso, \{Philip T.\} and Jacqueline Cattan",

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AU - Heller, Loree C.

AU - Davis, Carisa R.

AU - Peak, K. Kealy

AU - Wingfield, David

AU - Cannons, Andrew C.

AU - Amuso, Philip T.

AU - Cattan, Jacqueline

PY - 2003/3/1

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N2 - In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

AB - In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

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U2 - 10.1128/AEM.69.3.1844-1846.2003

DO - 10.1128/AEM.69.3.1844-1846.2003

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JO - Applied and Environmental Microbiology

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Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Abstract

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