Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Loree C. Heller; Carisa R. Davis; K. Kealy Peak; David Wingfield; Andrew C. Cannons; Philip T. Amuso; Jacqueline Cattan

doi:10.1128/AEM.69.3.1844-1846.2003

Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Loree C. Heller, Carisa R. Davis, K. Kealy Peak, David Wingfield, Andrew C. Cannons, Philip T. Amuso, Jacqueline Cattan

Biology

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Original language	English
Pages (from-to)	1844-1846
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	69
Issue number	3
DOIs	https://doi.org/10.1128/AEM.69.3.1844-1846.2003
State	Published - 1 Mar 2003

Access to Document

10.1128/AEM.69.3.1844-1846.2003

Cite this

@article{973d42c81cc640ccbfcd6d290b38379c,

title = "Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR",

abstract = "In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.",

author = "Heller, {Loree C.} and Davis, {Carisa R.} and Peak, {K. Kealy} and David Wingfield and Cannons, {Andrew C.} and Amuso, {Philip T.} and Jacqueline Cattan",

year = "2003",

month = mar,

day = "1",

doi = "10.1128/AEM.69.3.1844-1846.2003",

language = "English",

volume = "69",

pages = "1844--1846",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "3",

}

TY - JOUR

T1 - Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

AU - Heller, Loree C.

AU - Davis, Carisa R.

AU - Peak, K. Kealy

AU - Wingfield, David

AU - Cannons, Andrew C.

AU - Amuso, Philip T.

AU - Cattan, Jacqueline

PY - 2003/3/1

Y1 - 2003/3/1

N2 - In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

AB - In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

UR - http://www.scopus.com/inward/record.url?scp=0037335813&partnerID=8YFLogxK

U2 - 10.1128/AEM.69.3.1844-1846.2003

DO - 10.1128/AEM.69.3.1844-1846.2003

M3 - Article

C2 - 12620880

AN - SCOPUS:0037335813

SN - 0099-2240

VL - 69

SP - 1844

EP - 1846

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 3

ER -

Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Abstract

Access to Document

Other files and links

Fingerprint

Cite this