A cell-based fluorescent assay to detect the activity of AB toxins that inhibit protein synthesis

Patrick Cherubin, Beatriz Quiñones, Salem Elkahoui, Wallace Yokoyama, Ken Teter

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages25-36
Number of pages12
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1600
ISSN (Print)1064-3745

Keywords

  • AB toxin
  • Ricin
  • Shiga toxin
  • Toxicity assay
  • Toxin detection
  • Toxin inhibitors
  • Vero cells

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